Integrated phenotypic profiling and therapeutic target prioritization of clinical MDR Pseudomonas aeruginosa with biofilm-driven persistence

Fifteen clinical MDR P. aeruginosa isolates were profiled for susceptibility and biofilm formation. Persistence-associated target PA0328 (AaaA) was modelled and docked against eight ligands; pimozide and EGCG ranked highest with distinct binding mechanisms. ADMET favoured pimozide for repurposing potential. The scalable framework integrates phenotypic, structural, and pharmacokinetic dimensions for target prioritization.